Peek-A-Boo nursery on Cambridge Street was targeted on July 28 and again on August 17.
On the first occasion, burglars smashed two windows to get inside the nursery’s office, making off with around £200 in cash which staff members had saved for their Christmas party.
Three weeks later thieves stole £225 which had been raised to buy the nursery a water dispenser. A laptop was also stolen.
Nursery manager Natalie Pollard described it as “scary” that somebody would want to target a nursery repeatedly.
She said: “We have been here 12-and-a-half years and haven’t had any problems.
“We thought it was a one-off the first time they broke in, coming in to grab a bit of loose cash.”
Both incidents occured in the early hours, with the intruders tearing the nursery’s security alarm from the wall during the second theft using a large plank of wood.
They then forced their way through the front door and into the nursery’s office.
“It was very upsetting when we found out,” said Natalie, who has worked at Peek-A-Boo since 2006.
“We are a private nursery and so we put a lot of effort into fundraising events.
“It is scary to think somebody would target us like this when we don’t really have anything here.”
CCTV images showed one of the intruders gaining access to the front door.
Anybody with information should contact the police on 101 or Crimestoppers on 0800 555111.